Journal: NeuroSci

Article Title: Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer’s Disease Brains

doi: 10.3390/neurosci3020013

Figure Lengend Snippet: The primary antibodies used in this study.

Article Snippet: TXNIP , TXNIP , Bethyl , Rabbit/Poly , A303-229A , WB , 1:10,000.

Techniques:

Journal: NeuroSci

Article Title: Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer’s Disease Brains

doi: 10.3390/neurosci3020013

Figure Lengend Snippet: Comparison of TXNIP recognition patterns with antibodies from different sources in different brain-derived cells. Parallel immunoblot membranes with the same amounts of cellular proteins were probed with optimal dilution of four commercial TXNIP antibodies. The results show varying recognition patterns. Equivalent loading between cellular samples is demonstrated with reprobing blots for β-actin. The blot showing results of using AbCAM TXNIP antibody had previously been probed with TXNIP Bethyl antibody Abbreviations: MG, extracts from control (Cont) and Aβ treated microglial extracts from different cases (1,2,3); EC, extracts from primary human brain-derived endothelial cells; MEC, extracts from transformed brain endothelial cell line hCMEC/D3; Astrocyte, extract from brain derived primary astrocytes; iPS-Neu, extracts from Cellular Dynamics iCell human stem cell-derived human neurons; LAN5, extracts from differentiated human neuroblastoma cell line LAN-5 control (Cont) or high-glucose treated (Glu); Brain, extract of human brain samples from AD middle temporal gyrus (MTG).

Article Snippet: TXNIP , TXNIP , Bethyl , Rabbit/Poly , A303-229A , WB , 1:10,000.

Techniques: Comparison, Derivative Assay, Western Blot, Control, Transformation Assay

Journal: NeuroSci

Article Title: Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer’s Disease Brains

doi: 10.3390/neurosci3020013

Figure Lengend Snippet: TXNIP and TRX levels in low-plaque (LP), high-plaque (HP), and Alzheimer’s disease (AD) cases. ( A ) Representatives immunoblot of LP, HP, and AD cases showing TXNIP and TRX bands simultaneously on the same membrane. Membranes were probed with rabbit anti-TXNIP (Bethyl, 1:10,000 dilution) and rabbit anti-TRX (Abcam, 1:5000 dilution). Membranes were subsequently reacted with HRP-labeled anti-β-actin antibody (1:15,000) for normalization of loading. ( B ) Relative levels of TXNIP in LP, HP, and AD middle temporal gyrus brain protein extracts. Relative intensities were normalized for β-actin levels. NS, non-significant differences between groups. ( C ) Relative levels of TRX detected in LP, HP, and AD middle temporal gyrus brain protein extracts. Relative intensities were normalized for β-actin levels. NS, non-significant difference between groups.

Article Snippet: TXNIP , TXNIP , Bethyl , Rabbit/Poly , A303-229A , WB , 1:10,000.

Techniques: Western Blot, Membrane, Labeling

Journal: BioMed research international

Article Title: Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway.

doi: 10.1155/2022/7690960

Figure Lengend Snippet: Figure 2: Effects of systemic hypoxia stimulation on the expression of TXNIP/NLRP3 signaling pathway-related factors in periodontal tissues of rats: (a) immunofluorescence staining (IF) showed that TXNIP, NLRP3, ASC, and caspase-1 in periodontal tissues. Ab: alveolar; PL: periodontal ligament; R: root. Scale bars = 25 μm. (b) RT-PCR analysis of TXNIP, NLRP3, ASC, caspase-1, and IL-1β in periodontal tissues (n = 5). (c) Western blotting analysis of TXNIP, NLRP3, ASC, caspase-1, and IL-1β in periodontal tissues (n = 5 for each group). The above data are presented as the mean ± SEM, ∗p < 0:05.

Article Snippet: For IF, the sections were incubated overnight at 4°Cwith primary antibodies (1 : 250) diluted in PBS: TXNIP (Rabbit IgG, Boster, China), NLRP3 (Rabbit IgG, Boster, China), ASC (Rabbit IgG, Immunoway, USA), and caspase-1 (Rabbit IgG, Immunoway, China) and then incubated with Alexa Fluor-488 and Alexa Fluor-555 conjugate secondary antibodies at room temperature for 1h.

Techniques: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Cell Death & Disease

Article Title: Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure

doi: 10.1038/s41419-019-1552-y

Figure Lengend Snippet: A list of antibodies used in the studies

Article Snippet: Rabbit anti-thioredoxin interacting protein (TXNIP) antibody , bs-3897R , Bioss, Beijing, China , 1:50.

Techniques:

Journal: NeuroSci

Article Title: Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer’s Disease Brains

doi: 10.3390/neurosci3020013

Figure Lengend Snippet: The primary antibodies used in this study.

Article Snippet: TXNIP , TXNIP (JY2) , Novus , Mouse/Mono , NBP1-54578 , IHC/WB , 1:200–500.

Techniques: